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| Silva,Tatiane M.; Stets,Maria I.; Mazzetto,André M.; Andrade,Fabiana D.; Pileggi,Sônia A. V.; Fávero,Paulo R.; Cantú,Marcelo D.; Carrilho,Emanuel; Carneiro,Paulo I.B.; Pileggi,Marcos. |
| The aim of this work was to isolate microorganisms from Brazilian soil contaminated with 2,4-D herbicide, and analyze the efficiency for 2,4D degradation, using high-performance liquid chromatography (HPLC). Serratia marcescens and Penicillium sp had never been reported as able to degrade 2,4-D. The isolated strains represent a great potential for bioremediation. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioremediation Biodegradation 2; 4-dichlorophenoxyacetic acid. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300026 |
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| Grabicoski,Edilaine Mauricia Gelinski; Jaccoud Filho,David de Souza; Pileggi,Marcos; Henneberg,Luciane; Pierre,Marcelo Luiz Cunha; Vrisman,Cláudio Mauricio; Dabul,Audrei Nisio Gebieluca. |
| Caused by Sclerotinia sclerotiorum, white mold is an important seed-transmitted disease of soybean (Glycine max). Incubation-based methods available for the detection and quantification of seed-borne inoculum such as the blotter test, paper roll and Neon-S assay are time-consuming, laborious, and not always sensitive. In this study, we developed and evaluated a molecular assay for the detection of S. sclerotiorum in soybean seeds using a species-specific PCR (polymerase chain reaction) primer set and seed soaking (without DNA extraction) for up to 72 h. The PCR products were amplified in all the samples infected with the pathogen, but not in the other samples of plant material or the other seed-borne fungi DNA. The minimum amount of DNA detected was 10 pg,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: White mold; Dissemination; Soaking seeds; Diagnostic. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100069 |
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| Martins,Paula Fabiane; Martinez,Camila Ortiz; Carvalho,Giselle de; Carneiro,Paulo Irajara Borba; Azevedo,Ricardo Antunes; Pileggi,Sônia Alvim Veiga; Melo,Itamar Soares de; Pileggi,Marcos. |
| The aim of this work was to study herbicide degradation through selected microorganisms from humus and soil subjected to different plantation systems. The following bacterial species were identified: Klebsiella pneumoniae pneumoniae GC s.B strain 1, Pseudomonas alcaligenes, Enterobacter aerogenes GC s.A and Klebsiella pneumoniae pneumoniae GC s.B strain 2. Growth studies yet suggested the possibility of a very long lag phase. Although, culture with the herbicide presented biofilm formation and there were color changes in the herbicide that could have interfered with the espectrophotometry readings. After 5 days of incubation at 35ºC, the difference in the concentration of herbicide was 14.42% on average and after 10 days, 35.01%. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioremediation; Biodegradation; S-Metolachlor; Herbicide. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100019 |
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